QUIENES SOMOS

CONTATO ON LINE

COMPUMEDICS

Equipos de Diagnóstico en las Áreas de Sueño, la Neurología y la Cardiología

NEUROSCAN

Softwares, Amplificadores
y Accesorios para la Investigación en Neurociencia

MEDOC

Sistemas de Neuro Diagnóstico para Pesquisa
da Dor Cônica e Aguda

ALPHA OMEGA

Equipos para investigación neurofisiológica en animales

○

º

º

º

○

º

º

º

○

º

○

º

º

º

º

º

º

UGO BASILE

Instrumentos para
Animales de Laboratorio

○

Equipos de
neurodiagnóstico:
EMG, PE y IOM

○

○

○

Equipos de
neurodiagnóstico:
EEG, MG, PE y IOM

○

○

○

○

○

○

-
-
-
-
-
-

○

○

○

○

BIOLOG

Sistema de Identificación Microbiana

○

○

○

○

○

○

○

AEROCRINE - BIOSCAN

Medidores Portátil de
Óxido Nítrico (FENO)

○

SLEEP SUPPORT

Sensores e electrodos para EEG, PSG, PE, EMG y ECG

○

Cinturones de Esfuerzo Respiratorio, Torácicos
y Abdominales

○

○

○

○

Electrodo de Superficie de Copa Orificada Reutilizable
Sueño - EEG - Mapeo

Rainbow™ Agar - Biolog

Salmonella Detection

Intended Use

For laboratory use as a selective, chromogenic culture medium to aid in the detection and isolation of H₂S producing Salmonella species. Not for human in vitro diagnostic use.

Summary of Test

The United States Department of Agriculture - Food Safety Inspection Service (USDA-FSIS) has instituted Salmonella pathogen reduction performance standards for the meat processing and packaging industry¹. Salmonella was targeted “because it is the primary cause of foodborne illness among enteric pathogens”¹. Rainbow Agar Salmonella utilizes an enhanced detection chemistry to determine H₂S production among Salmonella spp.. Black colonies are formed by even weak H₂S producing strains. In addition, novel selective agents increase the recovery rate of Salmonella while inhibiting the growth of other enteric bacteria and inhibiting H₂S production by Citrobacter and other H₂S positive enteric species.

Contents

This packet contains dry powder to make Rainbow Agar Salmonella. 47 grams of powder will make 1 liter or approximately 50 plates.

Preparation

To prepare 1 liter of Rainbow Agar Salmonella mix one 47 gram packet into 990 ml of purified water, add 10 ml of 35% glycerol. Boil until agar and other components are completely dissolved or autoclave for 5 minutes at 15 lbs. pressure and 121°C. DO NOT exceed 5 minutes at this heat and pressure. After autoclaving, the medium will be green and turbid. Cool agar to 45-50°C before pouring. Stir and dispense approximately 20 ml of media into each petri plate. The medium is ready to use as soon as it has cooled, gelled and the surface has dried. The final medium  should be transparent and light yellow in color.

No pH adjustment is needed. The final pH should be pH 7.2-7.6. For samples that have a high background count of enteric bacteria, 100 mg/L sodium selenite can be added to make the medium more selective.

Storage

Rainbow Agar Salmonella powdered medium is extremely hygroscopic and should be stored at 2°C to 25°C in a dry environment. Rainbow Agar Salmonella plates are stable for 12 weeks when stored under refrigeration and packaged in cellophane to inhibit moisture loss.

User Quality Control

The following organisms are recommended if quality control is desired or required. Inoculate Rainbow Agar Salmonella plates with the following strains by streaking for isolation, then incubate for 24 hours at 35°C without elevated CO₂. Colony color should be read from isolated colonies.

Procedure

Inoculate the medium by streaking or spreading a sample suspected of containing Salmonella on the surface of the medium. Incubate the plates for 20 to 24 hours at 35°C without elevated CO₂. Some strains may need longer incubation for color development. (See the expected results below). Incubation beyond 36 hours may result in the detection of other H₂S positive bacteria (e.g., Citrobacter). Salmonella colonies are detected by their distinctive black coloration. H₂S production may be concentrated at the edges of heavy growth with central areas gray or white. Isolated colonies will be black. If 100 mg/L sodium selenite is added to make the medium more selective, the background microbial growth may appear orange due to selenite reduction.

Expected Results^a

Limitations

This medium should not be used as the sole basis for identification of microorganisms. Any colony suspected of being Salmonella should be tested further to verify its identity using an approved confirmatory protocol.

Some Citrobacter strains will turn black if the plates are allowed to incubate 36 hours or longer. The pyrrolidonylamidase (PYR) spot test may be used to differentiate Salmonella spp. (PYR negative) from Citrobacter spp. (PYR positive).

References

Biolog Ordering Information

Rainbow Agar Salmonella

|  Productos Biolog  |  Gen III™  |  OmniLog™  |  EcoPlate™ | FF MicroPlate™ |  

BiolinkArg / Bioscan - Representante exclusivo de Biolog en Argentina